anti myhc type iib  (Developmental Studies Hybridoma Bank)

Journal: Skeletal Muscle

Article Title: Displaced myonuclei are attributable to both resident myonuclear migration and stem cell fusion during mechanical loading in adult skeletal muscle

doi: 10.1186/s13395-025-00407-0

Figure Lengend Snippet: Immunohistochemical representative images of the plantaris muscle for fibre type-specific quantification of displaced myonuclei for 3-day MOV ( A & A ’) and 7-day MOV ( B & B ’). Images show dystrophin (pink), myosin heavy chain IIb (MyHC IIb, red), GFP resident myonuclei (green), and DAPI nuclei (blue). Quantification of displaced myonuclei according to fibre type and condition is reported in C&D with the absolute number of myonuclei per cross-section in panel C and relative number of IIb + or IIb- fibres with one or more displaced myonuclei in panel D . Data are presented as Mean ± SEM and is reported relative to each respective fibre type. (* p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001,**** p ≤ 0.0001; 2-way ANOVA Treatment x Nuclei Label). MOV – mechanical overload, EdU – 5-ethynyl-2’-deoxyuridine, DYS – dystrophin, GFP – green fluorescent protein. Yellow arrows – GFP-, Green arrows – GFP+

Article Snippet: For fibre type assessments, sections were incubated in primaries for MyHC IIb (BF-F3, DSHB) and dystrophin then the appropriate secondary antibodies (isotype-specific for MyHC IIb) before applying DAPI.

Techniques: Immunohistochemical staining

Journal: Skeletal Muscle

Article Title: Displaced myonuclei are attributable to both resident myonuclear migration and stem cell fusion during mechanical loading in adult skeletal muscle

doi: 10.1186/s13395-025-00407-0

Figure Lengend Snippet: Frequency distributions show the occurrences of GFP+ (green) and EdU+ (white) displaced nuclei according to fibre cross sectional area ( A , C , E ). Panels B , D , & F show these frequencies as a proportion of total fibre counts on entire plantaris cross sections. Data are presented as Mean ± SEM and frequency distributions. (* p ≤ 0.05; 2-way ANOVA Treatment x Nuclei Label). MOV – mechanical overload, EdU – 5-ethynyl-2’-deoxyuridine, CSA – cross sectional area, DYS – dystrophin, GFP – green fluorescent protein, MyHC IIb – myosin heavy chain type IIb

Article Snippet: For fibre type assessments, sections were incubated in primaries for MyHC IIb (BF-F3, DSHB) and dystrophin then the appropriate secondary antibodies (isotype-specific for MyHC IIb) before applying DAPI.

Techniques:

Journal: Cells

Article Title: C9ORF72 Is Pivotal to Maintain a Proper Protein Homeostasis in Mouse Skeletal Muscle

doi: 10.3390/cells14221765

Figure Lengend Snippet: Loss of C9orf72 induces muscle atrophy and alterations in the muscle fiber composition. Representative optical images of the GCM muscle in C9 +/+ ( A ) and C9 −/− ( B ) mice and myofibers mean cross-sectional area (CSA) distribution ( C ) in both experimental groups. Representative images of coronal sections of the GCM muscle in C9+/+ ( D ) and C9 −/− ( E ) labeled with laminin (white), myosin heavy chain (MyHC) type I (red), IIA (green), IIX (black), and IIB (blue). Analysis of the fiber type composition is shown in ( F ). Type IIX fibers correspond to unlabeled fibers. Representative electron micrographs of C9 +/+ ( G ) and C9 −/− ( H ) GCM muscles, respectively. * = slow (S) motor units. Data are expressed as mean± SEM of six mice per group and examined by two-way ANOVA followed by Fisher’s LSD test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: To determine the fiber type, the sections were incubated with MyHC type I (BA-D5, 1:10; DSHB, Iowa City, IA, USA), MyHC type IIa (SC-71, 1:17; DSHB, Iowa City, IA, USA), MyHC type IIb (BF-F3, 1:9; DSHB, Iowa City, IA, USA), Rabbit anti-Laminin (1:100, L9393; Sigma-Aldrich, St. Louis, MO, USA) primary antibodies and respective secondary antibodies, anti-MIgG2b Alexa-flour 564 (A21144, 1:500) (Invitrogen, Waltham, MA, USA), anti-MIgG1 Alexa-flour 488 (A21121, 1:500) (Invitrogen, Waltham, MA, USA), anti-MIgM Alexa-flour 647 (A21046, 1:500; Invitrogen, Waltham, MA, USA), and anti-Rabbit Alexa Fluor 405 (ab175649, 1:500; Abcam, Cambridge, UK).

Techniques: Labeling, Muscles